Fmoc-Ile-OH CAS 71989-23-6

Fmoc-Ile-OH is a fluorenylmethyloxycarbonyl-protected isoleucine — a critical amino acid building block used in solid-phase peptide synthesis (SPPS) and custom peptide production. The Fmoc protecting group ensures base-labile protection of the α-amino group, enabling stepwise chain elongation with high efficiency and minimal side reactions.

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Product Introduction

Fluorenylmethyloxycarbonyl-Protected Isoleucine | Solid-Phase Peptide Synthesis (SPPS) Building Block

Fmoc-Ile-OH is a fluorenylmethyloxycarbonyl-protected isoleucine - a critical amino acid building block used in solid-phase peptide synthesis (SPPS) and custom peptide production. The Fmoc protecting group ensures base-labile protection of the α-amino group, enabling stepwise chain elongation with high efficiency and minimal side reactions.

This high-quality Fmoc-amino acid is widely used in peptide synthesis facilities, contract research organizations (CROs), and pharmaceutical peptide manufacturing.

Chemical Name	Fluorenylmethyloxycarbonyl Isoleucine
Short Name	Fmoc-Ile-OH
CAS Number	71989-23-6
Molecular Formula	C₂₁H₂₁NO₄
Molecular Weight	351.39 g/mol
Appearance	White to off-white crystalline powder
Purity	≥99.0% (HPLC)
Solubility	Soluble in DMF, DCM, NMP
Grade	Peptide synthesis grade
Storage	2–8°C, dry, protect from light
Shelf Life	≥24 months (proper storage)

What Is Fmoc-Ile-OH?

Fmoc-Ile-OH is Isoleucine with an Fmoc protecting group (9-fluorenylmethyloxycarbonyl) on its α-amino function. The Fmoc group prevents undesired reactions during peptide chain assembly and is removed under mild basic conditions (typically 20% piperidine in DMF), allowing sequential addition of amino acids on solid support.

This protecting group strategy is the standard for Fmoc-based SPPS, a backbone of modern peptide synthesis workflow.

Fmoc-Ile-OH Key Features & Advantages

01/

High Purity for Reliable Synthesis

With ≥99% purity (HPLC validated), Fmoc-Ile-OH provides excellent reliability in generating high-quality peptides with minimum byproducts.

02/

Efficient Fmoc Removal

The Fmoc protecting group is easily cleaved under standard deprotection conditions, reducing cycle time and improving peptide chain fidelity.

03/

Excellent Compatibility

Suitable for use with:

Rink amide resin
Wang resin
Boc/Fmoc hybrid strategies
Automated peptide synthesizers

04/

Consistent Performance

Superior lot-to-lot consistency makes it ideal for:

Research labs

Peptide drug discovery

API peptide intermediates

Optimized production workflows

Fmoc-Ile-OH Applications

Fmoc-Ile-OH is an essential raw material for:

Solid-Phase Peptide Synthesis (SPPS)
Custom peptide libraries
Therapeutic peptide development
Biological and biochemical research
Peptide-based probe synthesis
Structure–function studies

Handling & Storage

Store at 2–8°C in a dry, dark environment.
Avoid moisture and prolonged exposure to light to preserve purity.
Use proper PPE (gloves, goggles) and follow institutional safety protocols.
Refer to the SDS for safe handling, disposal, and hazard information.

FAQ

Q1: What is the role of the Fmoc protecting group?
A1: It protects the amino moiety during peptide chain assembly and is removed under mild basic conditions.

Q2: How does Fmoc-Ile-OH differ from Boc-Ile-OH?
A2: Fmoc provides base-labile protection (milder, widely used), whereas Boc is acid-labile (strong acid required for deprotection).

Q3: Can it be used in automated peptide synthesizers?
A3: Yes, Fmoc-Ile-OH is fully compatible with automated Fmoc-SPPS machines.

Q4: What solvents should be used for coupling?
A4: Commonly used solvents include DMF, DCM, and NMP, often in the presence of coupling reagents like HBTU/HATU.

Safety & Disclaimer

Fmoc-Ile-OH is a chemical reagent intended for laboratory and industrial peptide synthesis applications only.
Not for human, animal, or medical use. Follow all relevant safety, regulatory, and waste disposal guidelines.